Fermentation of Enset (Ensete ventricosum) in the Gamo Highlands of Ethiopia: Introduction of Sauerkraut Jars

Ellen Gorrens
Persbericht

Zuurkoolpotten in de strijd voor ‘de boom tegen honger’

KochoMeer dan 15 miljoen Ethiopiërs zijn afhankelijk van de veelzijdige, inheemse plant enset die de bijnaam ‘de boom tegen honger’ draagt. De lokale bevolking gebruikt z’n knol en pseudostam om te fermenteren tot het eindproduct kocho. Er zijn echter veel vraagtekens over de veiligheid en de kwaliteit van kocho. In kader van een VLIR-UOS project, vertrok Ellen Gorrens richting de Ethiopische Hooglanden ter verbetering van het traditionele fermentatieproces.

Momenteel worden in de Ethiopische Hooglanden putten en bamboemanden gebruikt om het plantenmateriaal te fermenteren. Het gebruik ervan leidt tot slechte kocho en de aanwezigheid van insecten. Desondanks heeft het fermentatieproces amper aandacht gekregen van onderzoekers. Omwille van die beperkte kennis in de literatuur, was het noodzakelijk om spontane fermentaties te karakteriseren. Daarvoor werden naast de traditionele putten en bamboemanden ook zuurkoolpotten gebruikt. De aanwezigheid van een glazuurlaag en een waterslot zorgen ervoor dat de pot volledig (water)dicht is. Dat kan leiden tot een beter gecontroleerd proces met een betere kochokwaliteit.

Resultaten

De fermentatie duurt zo’n twee à drie maanden. Gedurende die tijd zorgen melkzuurbacteriën voor een scherpe daling in pH, wat voor een langere houdbaarheid zorgt. Na 90 dagen fermenteren bleek de pH in de zuurkoolpotten effectief het laagste te zijn. Die lage pH zorgde ervoor dat Enterobacteriaceae (een familie bacteriën vol ziekteverwekkers) geen kans meer hebben in de kocho. Clostridium sporen, die aanleiding kunnen geven tot ziektes, waren aanwezig in hoge aantallen gedurende heel de fermentatie, wat vragen doet rijzen over de voedselveiligheid. De aantallen in de zuurkoolpotten waren wel consistent lager dan in de andere systemen. Dat is een voordeel aangezien de lokale bevolking soms onvolledige gefermenteerde kocho consumeert in het geval van een voedseltekort. Bovendien werd de kocho komende van de zuurkoolpotten bestempeld door 50 proefpersonen als beste qua geur, hardheid, smaak en algemene acceptatie.

ToekomstLokaal gemaakte zuurkoolpotten

Zuurkoolpotten zijn dus een beter fermentatiesysteem dan de putten en bamboemanden. Ze zorgen ervoor dat de kocho veiliger is en van betere kwaliteit. De aankoop van geïmporteerde zuurkoolpotten door Ethiopische families is evenwel financieel onmogelijk. De vervaardiging van lokaal gemaakte zuurkoolpotten kan soelaas brengen, en aangezien enset meestal in groep wordt verwerkt, is de oprichting van een duurzame coöperatie zeker een haalbare kaart. De lokaal gemaakte zuurkoolpotten hebben echter geen glazuurlaag. Verder onderzoek naar de haalbaarheid van die potten is dus eerst nodig. De eerste steen ter verbetering van het hoofdvoedsel van meer dan 15 miljoen Ethiopiërs is met deze wel gezet.

Bibliografie

Abebe, T., Wiersum, K., & Bongers, F. (2010). Spatial and temporal variation in crop diversity in agroforestry homegardens of southern Ethiopia. Agroforest Systems, 78(3), 309-322.

Abebe, Y., Bogale, A., Hambidge, K. M., Bailey, K., & Gibson, R. S. (2007). Phytate, zinc, iron and calcium content of selected raw and prepared foods consumed in rural Sidama, Southern Ethiopia, and implications for bioavailability. Journal of Food Composition and Analysis, 20(3), 161-168.

Abebe, Y., Stoecker, B., Hinds, M., & Gates, G. (2006). Nutritive value and sensory acceptability of corn- and kocho-based foods supplemented with legumes for infant feeding in southern Ethiopia. African Journal of Food Agriculture Nutrition and Development, 6(1), 1-19.

Adams, M. R. (1999). Safety of industrial lactic acid bacteria. Journal of Biotechnology, 68(2), 171-178.

Agren, G., & Gibbson, R. S. (1969). Food Composition Table for Use in Ethiopia I, Children’s Nutrition. Addis Ababa: Ethiopia and Institute of Medical Chemistry, University of Uppsala, Sweden.

Amede, T., Stroud, A., & Aune, J. (2004). Advancing human nutrition without degrading land resources through modeling cropping systems in the Ethiopian Highlands. Food and Nutrition Bulletin, 25(4), 344-353.

Andeta, A. F., Vandeweyer, D., Eshetu, F., Vancampenhout, K., Woldesenbet, F., & Van Campenhout, L. (2017). Traditional enset (Ensete ventricosum) fermentation in the Gamo highlands of Ethiopia: physiochemical and microbial dynamics. Proceedings of the Twenty-second Conference on Food Microbiology: 2017-09-21.

Andeta, A. F., Vandeweyer, D., Woldesenbet, F. C., Lievens, B., Vancampenhout, K., & Van Campenhout, L. (2018). Fermentation of enset (Ensete ventricosum) in the Gamo highlands of Ethiopia: practices and microbial community dynamics. Food Microbiology, 73, 342-350.

AOAC. (1990). Official methods of analysis of the Association of Official Analytical Chemists. Arlington: Association of Official Analytical Chemists, Inc.

Arthey, D., & Dennis, C. (1991). Vegetable processing. New York: VCH Publishers.

Arthur, J. W. (2014). Culinary Crafts and Foods in Southwestern Ethiopia: An Ethnoarchaeological Study of Gamo Groundstones and Pottery. African Archaeological Review, 31(2), 131-168.

Ashenafi, M. (2006). A review on the microbiology of indigenous fermented foods and beverages of Ethiopia. Ethiopian Journal of Biological Sciences, 5(2), 189-245.

Ashenafi, M., & Abebe, Y. (1996). Microbial load and incidence of Staphylococcus aureus in market Bulla and Kotcho, traditional Ethiopian processed food products from Enset (Ensete ventricosum). Ethiopian Journal of Health Development, 10(2), 117-122.

Assefa, A., & Fitamo, D. (2016). The Ethno Botanical Study and Distribution Patterns of Enset Landraces (Ensete ventricosum (Welw) Cheesman) in Aleta Chuko Districs, Sidamo Zone, South Nation Nationality People and Regional State, Ethiopia. Research & Reviews: Research Journal of Biology, 4, 1-11.

Assefa, E. (2002). Some aspects of watershed management—a case study of Hare River Basin, south Rift Vally of Ethiopia. Ethiopian Journal of Water Science and Technology, 5(1), 61-67.

Atlabachew, M., & Chandravanshi, B. S. (2008). Levels of major, minor and trace elements in commercially available enset (Ensete ventricosum (Welw.) Cheesman) food products (Kocho and Bulla) in Ethiopia. Journal of Food Composition and Analysis, 21(7), 545-552.

Baker, R., & Simmonds, N. (1953). The Genus Ensete in Africa. Kew Bulletin, 8(3), 405-416.

Barrangou, R., Yoon, S.-S., Breidt, F. J., Fleming, H. P., & Klaenhammer, T. R. (2002). Identification and Characterization of Leuconostoc fallax Strains Isolated from an Industrial Sauerkraut Fermentation. Applied and Environmental Microbiology, 68(6), 2877-2884.

Battcock, M., & Azam-Ali, S. (1998). Fermented Fruits and Vegetables: a Global Perspective. Rome: FAO.

Beganovic, J., Kos, B., Pavunc, A. L., Uroic, K., Jokic, M., & Suskovic, J. (2014). Traditionally produced sauerkraut as source of autochthonous functional starter cultures. Microbiological Research, 169(7), 623-632.

Belitz, H., Grosch, W., & Schieberle, P. (2004). Food Chemistry. Berlin: Springer-Verlag Berlin Heidelberg.

Bender, D. A. (2014). Introduction to Nutrition and Metabolism: Fifth Edition. Boca Raton: CRC Press.
Bhunia, A. K. (2008). Foodborne Microbial Pathogens: Mechanisms and Pathogenesis. New York: Springer New York.

Bosha, A., Dalbato, A., Tana, T., Mohammed, W., Tesfaye, B., & Karlsson, L. (2016). Nutritional and chemical properties of fermented food of wild and cultivated genotypes of enset (Ensete ventricosum). Food Research International, 89, 806-811.

Brandt, S., Anita, S., Clifton, H., Terrence, M., Endale, T., Mulugeta, D., . . . Shiferaw, T. (1997). The "Tree Against Hunger": Enset-based Agricultural System in Ethiopia.

Bräse, S., Gläser, F., Kramer, C. S., Lindner, S., Linsenmeier, A. M., Masters, K. S., . . . Zhong, S. (2013). The Chemistry of Mycotoxins. London: Springer.

Breidt, F. (2004). A Genomic Study of Leuconostoc mesenteroides and the Molecular Ecology of Sauerkraut Fermentations. Journal of Food Science, 69(1), 30-33.

Brinkman, J. (2006). Proeven van success, sensorisch onderzoek: technieken, procedures en toepassingen. Houten: Keesing Noordervliet.

Bullerman, L. B., & Bianchini, A. (2007). Stability of mycotoxins during food processing. International Journal of Food Microbiology, 119(1), 140-146.

Busse, H., Kurabachew, H., Ptak, M., & Fofanah, M. (2017). A Food-Based Approach to Reduce Vitamin A Deficiency. African Journal of Food Agriculture, Nutrition and Development, 17(3), 12227-12243.

Carvalho, B. F., Avila, C. L., Bernardes, T. F., Pereira, M. N., Santos, C., & Schwan, R. F. (2017). Fermentation profile and identification of lactic acid bacteria and yeasts of rehydrated corn kernel silage. Journal of Applied Microbiology, 122(3), 589-600.

Champion, J. (1967). Les bananiers et leur culture botanique et génétique. Paris: Institut Français de recherches fruitière outre-mer (I.F.A.C.).

Chen, H. (2013). Modern Solid State Fermentation: Theory and Practice. Dordrecht: Springer Netherlands.

Cho, K. M., Math, R. K., Asraful Islam, S. M., Lim, W. J., Hong, S. Y., Kim, J. M., . . . Yun, H. D. (2009). Novel multiplex PCR for the detection of lactic acid bacteria during kimchi fermentation. Molecular and Cellular Probes, 23(2), 90-94.

Commision Regulation (Ec) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. (2006, 20 december). Official Journal of the European Union.

Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs. (2005, december 12). Official Journal of the European Union.

Cotter, P. D., Hill, C., & Ross, R. P. (2005). Bacteriocins: Developing Innate Immunity for Food. Nature Reviews Microbiology, 3(10), 777-788.

CSA. (2011). Report on area and production of major crops. Addis Ababa: The Federal Democratic Republic of Ethiopia Central Statistical Agency.

Daba, T., & Shigeta, M. (2016). Enset (Ensete ventricosum) Production in Ethiopia: its Nutritional and Socio-Cultural Values. Agriculture and Food Sciences Research, 3(2), 66-74.

Daeschel, M. A., & Nes, I. F. (1995). Lactobacillus plantarum: Physiology, Genetics and Applications in Foods. In Y. H. Hui, & G. G. Khachatourians, Food Biotechnology: Microorganisms (pp. 721-742). Weinhem: Wiley-VCH.

Demissie, T., Ali, A., Mekonen, Y., Haider, J., & Umeta, M. (2010). Magnitude and distribution of vitamin A deficiency in Ethiopia. Food and Nutrition Bulletin, 31(2), 234-241.

D'Este, M., Alvarado-Morales, M., & Angelidaki, I. (2017). Amino acids production focusing on fermentation technologies – A review. Biotechnology Advances, 35(5), 1-12.

Dijk, R., Van den Berg, D., Beumer, R. R., de Boer, E., Dijkstra, A. F., Mout, L., . . . in 't Veld, S. (2015). Microbiologie van de Voedingsmiddelen: Methoden, principes en criteria. Nederland: MYbusinessmedia.

EFSA. (2007). Introduction of a Qualified Presumption of Safety (QPS) approach for assessment of selected microorganisms referred to EFSA. The EFSA Journal, 5(12), 1-16.

EHNRI. (1997). Food composition table for use in Ethiopia. Part III. Addis Ababa: Ethiopian Health and Nutrition Research Institute.

Ergun, R., Lietha, R., & Hartel, R. W. (2010). Moisture and Shelf LIfe in Sugar Confections. Critical Reviews in Food Science and Nutrition, 50(2), 162-192.

FDA. (2015). Evaluation and definition of potentially hazardous foods - Chapter 3: Factors that influence microbial growth. Found on 10 November 2017 on: https://www.fda.gov/ downloads.food/guidanceregulation /ucm252447.pdf.

Gálvez, A., Burgos, M. G., López, R. L., & Pulido, R. P. (2014). Food Biopreservation. New York: SpringerBriefs in Food, Health, and Nutrition.

Garedew, B., Ayiza, A., Haile, B., & Kasaye, H. (2017). Indigenous knowledge of Enset (Ensete ventricosum (Welw.) Cheesman) cultivation and management practice by Shekicho people, Southwest Ethiopia. Journal of Plant Sciences, 5(1), 6-18.

Gashe, B. (1987a). Kocho fermentation. Journal of Applied Bacteriology, 62, 473-477.

Gashe, B. A. (1987b). Spoilage organisms of kocho. Mircen Journal of applied microbiology and biotechnology, 3, 67-74.

Gibbs, P. (2002). Characteristics of spore-forming bacteria. In C. Blackburn, & P. J. McClure, Foodborne pathogens: Hazards, risk analysis and control (pp. 417-435). Cambridge: Woodhead Publishing.

Giday, M., Asfaw, Z., & Woldu, Z. (2010). Ethnomedicinal study of plants used by Sheko ethnic group of Ethiopia. Journal of Ethnopharmacology, 132(1), 75-85.

He, Q., & Chen, H. (2013). Pilot-Scale Gas Double-Dynamic Solid-State Fermentation for the Production of Industrial Enzymes. Food and Bioprocess Technology, 6(10), 2916-2924.

Holzapfel, W. (2002). Appropriate starter culture technologies for small-scale fermentation in developing countries. International Journal of Food Microbiology, 75(3), 197-212.

Holzapfel, W., Schillinger, U., & Buckenhüskes, H. J. (2003). Sauerkraut. In E. R. Farnworth, Handbook of Fermented Functional Foods (pp. 343-360). Boca Raton: CRC Press.

Huffnagel, H. P. (1961). A Report of the Agriculture in Ethiopia. Rome: FAO.

Hunduma, T. (2010). Research Experience and Achievements on Traditional Enset (Ensete ventricosum) Fermentation Process. Enset National Workshop. Wolkite, Ethiopia.

Hunduma, T., & Ashenafi, M. (2011a). Effect of altitude on microbial succesion during traditional enset (Ensete ventricosum) fermentation. International Journal of Food, Nutrition and Public Health, 4(1), 39-51.

Hunduma, T., & Ashenafi, M. (2011b). Traditional Enset (Ensete ventricosum) processing techniques in some parts of West Shewa Zone, Ethiopia. Journal of Agricultural Development, 2(1), 37-57.

Hunduma, T., Sadessa, K., Hilu, E., & Oli, M. (2015). Evaluation of Enset Clones Resistance against Enset Bacterial Wilt Disease (Xanthomonas campestris pv. musacearum). Veterinary Science & Technology, 6(3), 1-7.

Jay, M. J., Loessner, M. J., & Golden, D. A. (2005). Modern Food Microbiology Seventh Edition. New York: Springer Science.

Johanningsmeier, S., McFeeters, R. F., Fleming, H. P., & Thompson, R. L. (2007). Effects of Leuconostoc mesenteroides Starter Culture on Fermentation of Cabbage with Reduced Salt Concentrations. Journal of Food Science, 72(5), 166-172.

Johnsson, A. (1991). Growth of Clostridium tyrobutyricum during fermentation and aerobic deterioration of grass silage. Journal of the Science of Food and Agriculture, 54, 557-568.

Jung, J. Y., Lee, S. H., Lee, H. J., Seo, H.-Y., Park, W.-S., & Jeon, C. O. (2012). Effects of Leuconostoc mesenteroides starter cultures on microbial communities and metabolites during kimchi fermentation. International Journal of Food Microbiology, 153(3), 378-387.

Karssa, T. H., Ali, K. A., & Gobena, E. N. (2014). The microbiology of Kocho: An Ethiopian Traditionally Fermented Food from Enset (Ensete ventricosum). International Journal of Life Sciences, 8(1), 7-13.

Kjos, M., Nes, I. F., & Diep, D. B. (2009). Class II one-peptide bacteriocins target a phylogenetically defined subgroup of mannose phosphotransferase systems on sensitive cells. Microbiology, 155(9), 2949-2961.

König, H., & Berkelmann-Löhnertz, B. (2009). Maintenance of Wine-Associated Microorganisms. In H. König, G. Unden, & J. Fröhlich, Biology of Microorganisms on Grapes, in Must and in Wine (pp. 451-468). Berlin: Springer Berlin Heidelberg.

König, H., & Fröhlich, J. (2009). Lactic Acid Bacteria. In H. König, G. Unden, & J. Fröhlich, Biology of Microorganisms on Grapes, in Must and in Wine (pp. 3-4). Berlin: Springer-Verlag Berlin Heidelberg.

Kung, L. (2001). Silage Fermentation and Additives. Direct-fed Microbial, Enzyme & Forage Additive Compendium.

Lacerda, I. C., Miranda, R. L., Borelli, B. M., Nunes, A. C., Nardi, R. M., Lachance, M.-A., & Rosa, C. A. (2005). Lactic acid bacteria and yeatst associated with spontaneous fermentations during the production of sour cassava starch in Brazil. International Journal of Food Microbiology, 105(2), 213-219.

Landbouwcentrum voor voedergewassen. (2015). Mycotoxinen: een sluipend gevaar. Found on 5 March on: http://www.lcvvzw.be/wp-content/uploads/2015/09/Mycotoxinen_een_ sluipend_gevaar_voor_melkvee.pdf.

Lawless, H. T., & Heymann, H. (2010). Sensory Evaluation of Food: Principles and Practices. New York: Springer New York.

Lefebvre, D., Gabriel, V., Vayssier, Y., & Fontagné-Faucher, C. (2002). Simultaneous HPLC Determination of Sugars, Organic Acids and Ethanol in Sourdough Process. Food Science and Technology, 35(5), 407-414.

Makkink, C. (2004). Mycotoxinen: normen en waarden. De molenaar: vakblad voor de graanverwerkende en diervoederindustrie, 107, 14-16.

McDonald, P., Henderson, A., & Heron, S. (1991). The Biochemistry of Silage: 2nd Edition. Marlow: Chalcombe Publications.

Million, T., Eshetu, A., & Endriase, G. (2003). Enset-Based Farming Systems of Masha Woreda Sheka Zone Report No. 51. Addis Ababa: Ethiopian Agricultural Research Organization, SNNPRS Agricultural Research Institue.

Mohammed, B., Martin, G., & Laila, M. (2013). Nutritive values of the drought tolerant food and fodder crop enset. African Journal of Agricultural Research, 8(20), 2326-2333.

Moroni, A. V., Arendt, E. K., & Dal Bello, F. (2011). Biodiversity of lactic acid bacteria and yeasts in spontaneously-fermented buckwheat and teff sourdoughs. Food Microbiology, 28(3), 497-502.

Motarjemi, Y. (2002). Impact of small scale fermentation technology on food safety in developing countries. International Journal of Food Microbiology, 75(3), 213-229.

Mu, T., Sun, H., & Liu, X. (2017). Potato Staple Food Processing Technology. Singapore: Springer Briefs in Food, Health, and Nutrition.

Narvhus, J. A., & Gadaga, T. H. (2003). The role of interaction between yeasts and lactic acid bacteria in African fermented milks: a review. International Journal of Food Microbiology, 86(1), 51-60.

Negash, A. (2001). Diversity and conservation of enset (Ensete ventricosum Welw. Cheesman) and its relation to household food and livelihood security in South-western Ethiopia. Wageningen: Wageningen Universiteit.

Negash, A., & Niehof, A. (2004). The significance of enset culture and biodiversity for rural household food and livelihood security in southwestern Ethiopia. 21(1), 61-71.

Nigatu, A. (1992). Lactic Acid Bacteria of Fermenting Tef Dough and Fermented Kocho and their Inhibitory Effect on Certain Food-borne Pathogens or Spoilage Organisms. Addis Ababa: Ethiopian Nutrition Institute.

Nigatu, A., & Gashe, B. (1998). Effect of heat treatment on the antimicrobial properties of tef dough, injera, kocho and aradisame and the fate of selected pathogens. World Journal of Microbiology & Biotechnology, 14(1), 63-69.

Nigatu, A., Gashe, B. A., & Ayele, T. (1997). Bacillus spp. from fermented tef dough and kocho: identity and role in the two Ethiopian fermented foods. Ethiopian Journal of Science, 20(1), 101-114.

Nurfeta, A., Eik, L. O., Tolera, A., & Sundstøl, F. (2008). Chemical composition and in sacco dry matter degradability of different morphological fractions of 10 enset (Ensete ventricosum) varieties. Animal Feed Science and Technology, 146(1), 55-73.

Nyambane, B., Thari, W. M., Wangoh, J., & Njage, P. M. (2014). Lactic acid bacteria and yeasts involved in the fermentation of amabere amaruranu, a Kenyan fermented milk. Food Science & Nutrition, 2(6), 692-699.

Oguntoyinbo, F. A., & Dodd, C. E. (2010). Bacterial dynamics during the spontaneous fermentation of cassava dough in gari production. Food Control, 21(3), 306-312.

Olango, T. M., Tesfaye, B., Catellani, M., & Pè, M. E. (2014). Indigenous knowledge, use and on-farm management of enset (Ensete ventricosum (Welw.) Cheesman) diversity in Wolaita, Southern Ethiopia. Journal of Ethnobiology and Ethnomedicine, 10, 41-58.

Oyewole, O. B. (2001). Characteristics and significance of yeasts' involvement in cassava fermentation for 'fufu' production. International Journal of Food Microbiology, 65, 213-218.

Pahlow, G., Muck, R. E., Driehuis, F., Oude Elferink, S., & Spoelstra, S. F. (2003). Microbiology of ensiling. In D. R. Buxton, R. E. Muck, & J. H. Harrison, Somage Science and Technology (pp. 31-93). American Society of Agronomy.

Palani, K., Harbaum-Piayda, B., Meske, D., Keppler, J. K., Bockelmann, W., Heller, K. J., & Schwarz, K. (2016). Influence of fermentation on glucosinolates and glucobrassicin degradation products in sauerkraut. Food Chemistry, 190, 755-762.

Pederson, C. S., & Albury, M. N. (1969). The sauerkraut fermentation. New York: Cornell University.

Peñas, E., Frias, J., Gomez, R., & Vidal-Valverde, C. (2010). High hydrostatic pressure can improve the microbial quality of sauerkraut during storage. Food Control, 21(4), 524-528.

Petrova, P., Petrov, K., & Stoyancheva, G. (2013). Starch-modifying enzymes of lactic acid bacteria - structures, properties, and applications. Starch/Stärke, 35(2), 34-47.

Pijls, L. T., Timmer, A. A., Wolde-Gebriel, Z., & West, C. E. (1995). Cultivation, Preparation and Consumption of Ensete (Ensete ventricosum) in Ethiopia. Journal of the Science of Food and Agriculture, 67(1), 1-11.

Plengvidhya, V., Breidt, F. J., Lu, Z., & Fleming, H. P. (2007). DNA Fingerprinting of Lactic Acid Bacteria in Sauerkraut Fermentations. Applied and Environmental Microbiology, 73(23), 7697-7702.

Price, C. E., Zeyniyev, A., Kuipers, O. P., & Kok, J. (2012). From meadows to milk to mucosa – adaptation of Streptococcus and Lactococcus species to their nutritional environments. FEMS Microbiology Reviews, 36(5), 949-971.

Pundir, R. K., & Jain, P. (2010). Changes in Microflora of Sauerkraut During Fermentation and Storage. World Journal of Dairy & Food Sciences, 5(2), 221-225.

Regassa, N., & Stoecker, B. J. (2012). Household food insecurity and hunger among households in Sidama district, southern Ethiopia. Public Health Nutrition, 15(7), 1276-1283.

Regulation (EC) No 1333/2008 of the European Parliament and of the Council of 16 December 2008 on food additives. (2008, 31 December). Official Journal of the Europian Union.

Russell, N. J., & Gould, G. W. (1991). Food Preservatives. New York: AVI Publishing.

Salminen, S., Gorbach, S., Lee, Y.-K., & Benno, Y. (2004). Human Studies on Probiotics: What is Scientifically Proven Today? In S. Salminen, A. von Wright, & A. Ouwenhand, Lactic Acid Bacteria: Microbiological and Functional Aspects (pp. 515-530). New York: Marcel Dekker.

Sandford, J., & Kasa, H. (1993). The effect of gender on resource contribution, decision making and influence: A comparison between enset, tef and maize. Addis Ababa: Proceedings of the workshop on Enset-based Sustainable Agriculture in Ethiopia.

Shank, R., & Ertiro, C. (1996). A linear model for predicting enset plant yield and assessment of Kocho production in Ethiopia. Addis Ababa: World Food Programme, Ministry of Agriculture, Southern Nations, Nationalities, Peoples' Regional State, UNDP Emergencies Unit for Ethiopia.

Shergill-Bonner, R. (2013). Micronutrients. Paediatrics and Child Health, 23(8), 331-336.

Simmonds, N. (1963). The Evolution of the Bananas. London: Longmans, Green, London.

Smeds, H. (1955). The Ensete planting culture of Eastern Sidamo, Ethiopia: the role of the ensete-plantations in the local economy and their significance in the cultural geography of Ethiopia. Helsinki: Acta Geographica.

Sperber, D. (1974). La notion d'ainesse et ses paradoxes chez Les Dorzé d'Ethiopie méridionale. Cahiers internationaux de Sociologie, 56, 63-78.

Sperber, W. H., & Doyle, M. P. (2009). Compendium of the Microbiological Spoilage of Foods and Beverages. New York: Springer.

Steinkraus, K. H. (1996). Handbook of Indigenous Fermented Foods. New York: Marcel Dekker, INC.

Suskovic, J., Kos, B., Beganovic, J., Pavunc, A. L., Habjanic, K., & Matosic, S. (2010). Antimicrobial Activity – The Most Important Property of Probiotic and Starter Lactic Acid Bacteria. Food Technology and Biotechnology, 48(3), 296-307.

Tamminen, M., Joutsjoki, T., Sjöblom, M., Joutsen, M., Palva, A., Ryhänen, E. L., & Joutsjoki, V. (2004). Screening of lactic acid bacteria from fermented vegetables by carbohydrate profiling and PCR-ELISA. Letters in Applied Microbiology, 39(5), 439-444.

Taye, B., & Asrat, F. (1996). The production and utilization of the genus ensete in Ethiopia. Economic botany, 20(1), 65-70.

Tedla, M., & Abebe, Y. (1994). Study of Enset Processing and Development of Enset Processing Tools in the Southern Region of Ethiopia. Awassa: ACA/NORAGRIC research collaboration project.

Temesgen, A., & Getasew, A. (2015). Partial substitution of Barely Malt by effective use of selected secondary starch crops in brewing technology by Saccharomyces Cerevisiae with a case example of Dashen Brewery. American Journal of Food Science and Technology, 3(1), 24-26.

The FAO Statistics Division. (2015). Hunger map, millennium development goal 1 and world food summit hunger targets.

Tsegaye, A. (2002). On indigenous production, genetic diversity and crop ecology of enset (Ensete ventricosum (Welw.) Cheesman). Wageningen: Wageningen Universiteit.

Tsegaye, A., & Struik, P. (2000). Influence of repetive transplanting and leaf pruning on dry matter and food production of enset (Ensete ventricosum Welw. (Cheesman)). Field Crops Research, 68(1), 61-74.

Tsegaye, A., & Struik, P. (2001). Enset (Ensete ventricosum (Welw.) Cheesman) kocho yield under different crop establishment methods as compared to yields of other carbohydrate-rich food crops. Netherlands Journal of Agricultural Science, 49(1), 81-94.

Tsegaye, A., & Struik, P. (2002). Analysis of enset (Ensete ventricosum) indigenous production methods and farm-based biodiversity in major enset-growing regions of southern Ethiopia. Experimental Agriculture, 38(3), 291-315.

Tsegaye, Z., & Gizaw, B. (2015). Community Indigenous Knowledge on Traditional Fermented Enset Product Preparation and Utilization Practice in Gedeo Zone. Journal of Biodiversity and Ecological Sciences, 5(3), 214-232.

Tsehaye, Y., & Kebebew, F. (2006). Diversity and cultural use of Enset (Enset ventricosum (Welw.) Cheesman) in Bonga in situ Conservation Site, Ethiopia. Ethnobotany Research & Applications, 4, 147-157.

United States Agency International Development. (2005). Ethiopia Southern Nations, Nationalities and Peoples Region (SNNPR) Livelihood Zone Reports: SNNPR Follow-On to Regional Livelihoods Baseline Study.

Urga, K., Fite, A., & Biratu, E. (1997). Natural fermentation of enset (Ensete ventricosum) for the production of kocho. The Ethiopian Journal of Health Development, 11(1), 75-81.

Väre, H., & Häkkinen, M. (2011). Typification and check-list of Ensete Horan. names (Musaceae) with nomenclatural notes. Adansonia, 33(2), 191-200.

Vavilov, N. (1951). The Origin, Variation, Immunity, and Breeding of Cultivated Plants. Chronica Botanica, 13, 1-364.

Viander, B., Mäki, M., & Palva, A. (2003). Impact of low salt concentration, salt quality on natural large-scale sauerkraut fermentation. Food Microbiology, 20(4), 391-395.

Wareing, P., Stuart, F., & Fernandes, R. (2011). Factors affecting the growth of micro-organisms in foods. Softcover, 437.

Weinberg, Z. G. (2008). Preservation of Forage Crops by Solid-State Lactic Acid Fermentation-Ensiling. In A. Pandey, C. R. Soccol, & C. Larroche, Current Developments in Solid-State Fermentation (pp. 443-467). New York: Springer New York.

Westphal, E. (1975). Agricultural systems in Ethiopia. Wageningen: Centre for Agricultural Publishing and Documentation.

Wheeldon, L. J., Worthington, T., Hilton, A. C., Elliott, T. S., & Lambert, P. A. (2008). Physical and chemical factors influencing the germination of Clostridium difficile spores. Journal of Applied Microbiology, 105(6), 2223-2230.

WHO, & FAO. (2004). Vitamin and mineral requirements in human nutrition, Second edition. Geneva.

WHO/FAO/UNU Expert Consultation. (2007). Protein and Amino Acid Requirements in Human Nutrition. Geneva: WHO.

Yemataw, Z., Mekonen, A., Chala, A., Tesfaye, K., Mekonen, K., Studholme, D. J., & Sharma, K. (2017). Farmers' knowledge and perception of enset Xanthomonas wilt in southern Ethiopia. Agriculture & Food Security, 6(62), 1-12.

Yemataw, Z., Mohamed, H., Diro, M., Addis, T., & Blomme, G. (2014). Enset (Ensete ventricosum) clone selection by farmers and their cultural practices in southern Ethiopia. Genetic Resources and Crop Evolution, 61(6), 1091-1104.

Yemataw, Z., Tesfaye, K., Zeberga, A., & Blomme, G. (2016). Exploiting indigenous knowledge of subsistence farmers' for the management and conservation of Enset (Ensete ventricosum (Welw.) Cheesman) (musaceae family) diversity on-farm. Journal of Ethnobiology and Ethnomedicine, 12(34), 1-25.

Yirmaga, M. T. (2013). Improving the indigenous processing of kocho, an Ethiopian traditional fermented food. Journal of Nutrition and Food Sciences, 3(1), 1-6.

Zhang, W., & Zhang, H. (2014). Genomics of Lactic Acid Bacteria. In H. Zhang, & Y. Cai, Lactic Acid Bacteria (pp. 205-206). Dordrecht: Springer Science + Businnes Media Dordrecht.

Zheng, Y., Yates, M., Aung, H., Cheng, Y.-S., Yu, C., Guo, H., . . . Jenkins, B. M. (2011). Influence of moisture content on microbial activity and silage quality during ensilage of food processing residues. Bioprocess and Biosystems, 34, 987-995.

Zippel, K. (2005). Diversity over time and space in enset landraces (Ensete ventricosum) in Ethiopia. African Biodiversity, 423-438.

Universiteit of Hogeschool
Master biowetenschappen: voedingsindustrie
Publicatiejaar
2018
Promotor(en)
Anneke De Smedt
Kernwoorden
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